Frequently asked questions

Q: Why should I choose the Phadebas test for my alpha-Amylase tests?

A: The test uses a well-defined substrate, produced under GMP conditions. The test is well described and acknowledged in the scientific litterature. The analytical performance of the test is very good, with f.ex. RSD values much better than normally expected for an enzymatic assay. Phadebas has been shown to work well with a host of very different samples and applications. The substrate is specific for alpha-amylase, hence it will not cross-react with enzymes that hydrolyse non-reducing terminal residues of starch, such as for example beta-Amylase or Invertase do. Furthermore, each kit contains a verified, batch specific standard curve for quantitative determination of the activity.

For the products in the Forensic portfolio - the Phadebas sheets are the only product in the market capable of localising hidden saliva stains independent of where the saliva was deposited.

Q: Does Amylase of animal origin give rise to false positives in forensic casework?

A: Peter Lamb at the FSS showed that saliva samples taken from sheep, horse, cow, goat, pig, cat and dog did NOT yield positive results with the Phadebas Forensic press test. A quantitative determination of alpha-Amylase, using Phadebas tablets, revealed that Amylase activities in these animals were more than a 1000 times lower than in human saliva.

Q: Where and How can I buy the products?

A: Please use the ordering section of this website.

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Q: Should I incubate for 15 min or 30 min when analyzing honey according to the IHC method?

A: You should always incubate for 30 minutes when analyzing honey with Phadebas Honey Diastase Test. The method of the International Honey Commission has unfortunately not been updated yet. After a change of blue dye to a less reactive type one must incubate longer to reach the same results as before.

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Q: Wasn't the Phadebas test sold by another Swedish company?

A: Yes, Pharmacia Diagnostics invented the Amylase Test in the 1960's and was the sole distributor until Dec. 31, 2005. In 2005, Magle AB acquired all rights to the Phadebas brand and products.

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Q: Is Phadebas Amylase Test CE marked for in-vitro diagnostic use?

A: No, Phadebas is no longer marketed for the IVD market. No changes are made to the products, but we do not maintain the necessary registrations for upholding the product's IVD status.

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Q: My spectrophotometer doesn't measure at 620 nm. What if I use 590 nm?

A: 620 nm is an absorption maxima for the chromophore of the blue dye we use in Phadebas. You will still obtain measurable results, but you will lose in sensitivity and presumably in precision. This may result in increased RSD values and if you are measuring low or very low amylase activities, we recommend using the specified wavelength.

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Q: Can I analyse turbid samples?

A: Samples are often slightly turbid but if the supernatant is turbid or opaque the reading of absorbance will be affected. It may be possible to add a flocculation chemical to precipitate large molecules like fats etc. Please contact info@phadebas.com for more help.

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Q: Can I use deionised water instead of distilled?

A: You need to validate this change for your application. Consistent enzymatic performance in terms of the assay's accuracy and precision/repeatability need to be confirmed before changing to deionised water. For the forensic press test however, any type of clean water can be used.

DSM

The center of the picture shows a DSM. In the picture, the DSM is actively stimulating the blood clotting process, one of many applications of DSMs. Read more about our hemostasis product at www.magle.se