Phadebas® PRO-1341, 5 dishes
€168.00
Article number 1341, each dish contains 40 µl sample wells and can be incubated for up to 16 to18 hours at up to 60 °C. Instructions for use are included in English.
Description
Phadebas® PRO is a product for detection and semi-quantification of endo-protease activity.
Could be used e.g. in quality control for protease residues in Food Production Industry and R&D.
A Phadebas Pro dish has 7 sample wells, one for negative control and 6 for samples or references.
Liquid samples can be analyzed, independent of composition, coloring and opacity.
Phadebas PRO is Ready to Use, Easy to Use, Easy to Evaluate and requires low work effort and equipment.
Phadebas® Safety Data Sheets
Phadebas® Instructions for Use
Q&A Phadebas® PRO
Q: Can I reuse the dish?
A: No, not even if there are non used wells.
Q:Why am I not getting any white phase?
A: The activity is below detection level.
– The sample does not contain any protease.
– The protease in the sample has been deactivated.
– No sample has been added.
Q: How should I dilute the concentrated protease solution before use as a reference?
A; Dilute your protease reference freshly with deionised water before application and
in general, they can be diluted 100 000- 1000 000 times.
Q: Can I run different proteases on the same plate?
A: Yes, if your samples contain the same type of protease and could be detected at the same temperature.
Q: Why do I get a white zone in the negative control?
A: Your pipette, pipette tips, water etc. is contaminated with protease.
Q: What should I do if a white zone interacts with other white zones?
A: Dilute your sample with deionised water and run your sample ones more. Remember dilution factor.
Q: Can I analyse opaque and colored samples?
A: Yes, Sample must be in the liquid form, if not dilute with deionised water.
Q: Why do I not get any detection of my diluted reference sample?
A: It is important that your reference samples are freshly prepared prior to use, because protease can lose activity over time.
Q: Can I incubate the dish for more than 16-18 hours?
A: Yes, but there is a risk of dehydration and cracking of the gel.
Q: Why do I get white zones not connected to the wells?
A; The dish has been contaminated with protease from undefined source.
The samples should be reanalysed.
Q; Could bacteria, yeast or mold grow on the dish?
A; No, there is a preservative added to the dish to avoid this.
Q; Is it possible to detect enzymes other than proteases?
A; No, this dish is specific for endo-protease detection only.